CCL5 anticorps

N° du produit ABIN296462

L’anticorps Mouton Polyclonal anti-CCL5 a été validé pour WB, ELISA, Neut et Func. Il convient pour détecter CCL5 dans des échantillons de Cat.

Aperçu rapide pour CCL5 anticorps (ABIN296462)

Antigène: CCL5 (Chemokine (C-C Motif) Ligand 5 (CCL5))

Reactivité: Cat

Hôte: Mouton

Clonalité: Polyclonal

Conjugué: Cet anticorp CCL5 est non-conjugé

Application: Western Blotting (WB), ELISA, Neutralization (Neut), Functional Studies (Func)

Détail du produit anti-CCL5 anticorps

Specificité: Selected for its ability to neutralize feline RANTES bioactivity.

Purification: Affinity purified

Immunogène: E. coli-derived rfeRANTES.

Isotype: IgG

Information d'application

Indications d'application: Approved: ELISA (0.5 - 1 μg/mL), Func, Neut, WB (0.1 - 0.2 μg/mL)

Usage: Suitable for use in ELISA, Western Blot, Neutralization. Neutralization of Feline CCL5 Bioactivity: The exact concentration of antibody required to neutralize rfeRANTES activity is dependent on the cytokine concentration, cell type, growth conditions and the type of activity studied. The Neutralization Dose50 (ND50) for this antibody is defined as that concentration of antibody required to yield one-half maximal inhibition of the cytokine activity on a responsive cell line, when that cytokine is present at a concentration just high enough to elicit a maximum response. The ND50 for this lot of anti-feline RANTES antibody was determined to be approximately 0.5-2.5 μg/mL in the presence of 80 ng/mL of rfeRANTES, using transfected BaF/3 cells in a chemotaxis assay. The specific conditions are described in the figure legends. Western Blot: 0.1-0.2 μg/mL with the appropriate secondary reagents to detect feline RANTES. The detection limit for rfeRANTES is approximately 1 ng/lane under non-reducing and reducing conditions. Direct ELISA: 0.5-1.0 μg/mL with the appropriate secondary reagents to detect feline RANTES. The detection limit for rfeRANTES is approximately 0.5 ng/well. In this format, this antibody shows less than 5 % cross-reactivity with rcrRANTES, rhRANTES, and rmRANTES.

Commentaires:

Target Species of Antibody: Cat

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: deionized water

Concentration: Lot specific

Buffer: Lyophilized.

Détails sur CCL5

Antigène: CCL5 (Chemokine (C-C Motif) Ligand 5 (CCL5))

Autre désignation: CCL5 / RANTES

Sujet: Name/Gene ID: CCL5
Family: Intercrine

Synonyms: CCL5, Chemokine (C-C motif) ligand 5, D17S136E, EoCP, T-cell specific protein p288, SCYA5, SISd, T cell-specific protein P228, Beta-chemokine RANTES, C-C motif chemokine 5, RANTES, SIS-delta, Small-inducible cytokine A5, T-cell-specific protein RANTES, TCP228

ID gène: 6352

UniProt: P13501

Pathways: Cellular Response to Molecule of Bacterial Origin, Regulation of G-Protein Coupled Receptor Protein Signaling, Smooth Muscle Cell Migration